An Introduction to the Diagnosis of Plant Disease by Chelston W. D Brathwaite

By Chelston W. D Brathwaite

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Two types of endo-PO (alkaline and neutral) were purified from this clone. They, like other reported POs of different origins, did not require Ca 2+ for their activities and had pH optima in the acidic range. Considering that plant tissues have acidic pH, PO may be active in plants even at lesser amounts compared with PL whose optimum pH is in the alkaline range. Like the E. , 1985). It may be interesting to study this analogy in more detail to elucidate the regulatory mechanism for PO synthesis.

1985). Since formation of free radicals was detected as the electron spin resonance absorption of the freeze-dried whole plant tissues (data not shown), it is highly possible that Table 2. The activities of PL and PNL in the macerated plant tissues. Eca strains (ECl, T29) and Ech strains (07, 012, EC183) were inoculated onto various plant tissues. The activity was expressed as the units/10 9 cells. Strains Enzyme 07 PL PNL PL PNL PL PNL PL PNL PL PNL 012 EC183 ECI T29 - no detectable activity.

1989, Insertion of cutinase gene into a wound pathogen enable it to infect intact host, Nature 342:446-448. , 1989, Elicitor-active components from French bean hypocotyls, Physiol. Mol. Pl. Palhol. 34:99-115. , 1986, Phytoalexin synthesis: The biochemical analysis of the induction process, Annu. Rev. Phytopathol. 24:235-264. K. , 1987, Structure of cutinase gene, cDNA, and the derived amino acid sequence from phytopathogenic fungi. Biochemistry 26:7883-7902. , 1955, Host-parasite interaction in flax rust: its genetics and other implications, Phytopathology, 45:680-685.

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